Reporter

Part:BBa_K4244060:Design

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-09)


Constitutive expression of sfGFP with OmpA secretion tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The assembly was performed using the loop assembly method (Pollak, 2008) therefore scars between the element are present but does not interfere with the expression of the gene. The start codon was also removed from sfGFP to allow fusion with N-termini signal peptide.


Source

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References